Binding of the 5'-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription.
Identifieur interne : 001D57 ( Main/Exploration ); précédent : 001D56; suivant : 001D58Binding of the 5'-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription.
Auteurs : Yong Wah Tan [Singapour] ; Wanjin Hong ; Ding Xiang LiuSource :
- Nucleic acids research [ 1362-4962 ] ; 2012.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (métabolisme), Animaux, Cellules HeLa, Cellules Vero, Humains, Lignée cellulaire, Motifs et domaines d'intéraction protéique, Mutation, Noyau de la cellule (métabolisme), Petites ribonucléoprotéines nucléaires (), Petites ribonucléoprotéines nucléaires (génétique), Petites ribonucléoprotéines nucléaires (métabolisme), Régions 5' non traduites, Réplication virale, Transcription génétique, Transport de protéines, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- génétique : Petites ribonucléoprotéines nucléaires, Virus du SRAS.
- métabolisme : ARN viral, Noyau de la cellule, Petites ribonucléoprotéines nucléaires.
- physiologie : Virus du SRAS.
- ARN viral, Animaux, Cellules HeLa, Cellules Vero, Humains, Lignée cellulaire, Motifs et domaines d'intéraction protéique, Mutation, Petites ribonucléoprotéines nucléaires, Régions 5' non traduites, Réplication virale, Transcription génétique, Transport de protéines.
English descriptors
- KwdEn :
- 5' Untranslated Regions, Animals, Cell Line, Cell Nucleus (metabolism), Chlorocebus aethiops, HeLa Cells, Humans, Mutation, Protein Interaction Domains and Motifs, Protein Transport, RNA, Viral (chemistry), RNA, Viral (metabolism), Ribonucleoproteins, Small Nuclear (chemistry), Ribonucleoproteins, Small Nuclear (genetics), Ribonucleoproteins, Small Nuclear (metabolism), SARS Virus (genetics), SARS Virus (physiology), Transcription, Genetic, Vero Cells, Virus Replication.
- MESH :
- chemical , chemistry : RNA, Viral, Ribonucleoproteins, Small Nuclear.
- chemical , genetics : Ribonucleoproteins, Small Nuclear.
- chemical , metabolism : RNA, Viral, Ribonucleoproteins, Small Nuclear.
- chemical : 5' Untranslated Regions.
- genetics : SARS Virus.
- metabolism : Cell Nucleus.
- physiology : SARS Virus.
- Animals, Cell Line, Chlorocebus aethiops, HeLa Cells, Humans, Mutation, Protein Interaction Domains and Motifs, Protein Transport, Transcription, Genetic, Vero Cells, Virus Replication.
Abstract
Coronaviruses RNA synthesis occurs in the cytoplasm and is regulated by host cell proteins. In a screen based on a yeast three-hybrid system using the 5'-untranslated region (5'-UTR) of SARS coronavirus (SARS-CoV) RNA as bait against a human cDNA library derived from HeLa cells, we found a positive candidate cellular protein, zinc finger CCHC-type and RNA-binding motif 1 (MADP1), to be able to interact with this region of the SARS-CoV genome. This interaction was subsequently confirmed in coronavirus infectious bronchitis virus (IBV). The specificity of the interaction between MADP1 and the 5'-UTR of IBV was investigated and confirmed by using an RNA pull-down assay. The RNA-binding domain was mapped to the N-terminal region of MADP1 and the protein binding sequence to stem-loop I of IBV 5'-UTR. MADP1 was found to be translocated to the cytoplasm and partially co-localized with the viral replicase/transcriptase complexes (RTCs) in IBV-infected cells, deviating from its usual nuclear localization in a normal cell using indirect immunofluorescence. Using small interfering RNA (siRNA) against MADP1, defective viral RNA synthesis was observed in the knockdown cells, therefore indicating the importance of the protein in coronaviral RNA synthesis.
DOI: 10.1093/nar/gks165
PubMed: 22362731
Affiliations:
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Le document en format XML
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protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronaviruses RNA synthesis occurs in the cytoplasm and is regulated by host cell proteins. In a screen based on a yeast three-hybrid system using the 5'-untranslated region (5'-UTR) of SARS coronavirus (SARS-CoV) RNA as bait against a human cDNA library derived from HeLa cells, we found a positive candidate cellular protein, zinc finger CCHC-type and RNA-binding motif 1 (MADP1), to be able to interact with this region of the SARS-CoV genome. This interaction was subsequently confirmed in coronavirus infectious bronchitis virus (IBV). The specificity of the interaction between MADP1 and the 5'-UTR of IBV was investigated and confirmed by using an RNA pull-down assay. The RNA-binding domain was mapped to the N-terminal region of MADP1 and the protein binding sequence to stem-loop I of IBV 5'-UTR. MADP1 was found to be translocated to the cytoplasm and partially co-localized with the viral replicase/transcriptase complexes (RTCs) in IBV-infected cells, deviating from its usual nuclear localization in a normal cell using indirect immunofluorescence. Using small interfering RNA (siRNA) against MADP1, defective viral RNA synthesis was observed in the knockdown cells, therefore indicating the importance of the protein in coronaviral RNA synthesis.</div></front></TEI><affiliations><list><country><li>Singapour</li></country></list><tree><noCountry><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name></noCountry><country name="Singapour"><noRegion><name sortKey="Tan, Yong Wah" sort="Tan, Yong Wah" uniqKey="Tan Y" first="Yong Wah" last="Tan">Yong Wah Tan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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